Measuring Functional Heterogeneity Among Single Cells


New methods to study heterogeneity at cellular resolution in complex tissues are rapidly transforming our understanding of cellular heterogeneity in human tissues and disease.  However, their reliance on measurements of steady-state abundance of DNA, RNA, and protein limits our ability to extract dynamic information from single cells. Dr. Jay Hesselberth at the University of Colorado has developed a novel method for measuring enzymatic activities simultaneously in thousands of single cells, a scale that had not been previously achievable. Rather than measuring the abundance of molecules, the lab directly measures enzymatic activities present in single cells by analyzing the conversion of substrates to products by single cell extracts in a high-throughput DNA sequencing experiment. This technology is compatible with existing platforms and can be tailored to measure many different enzymatic activities simultaneously.


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Mary Tapolsky
University of Colorado
Jay Hesselberth
Amanda Richer
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