CU investigators have generated a polyclonal antibody to detect mouse ORF1 protein, which is required for retrotransposition of the LINE-1 retrotransposon. The antibody was generated by immunizing rabbits with recombinant mouse ORF1 protein. Testing shows that the antibody is highly specific and capable of detecting 1 ng of protein in a western blot. In addition to use in western blotting, the antibody can be used immunofluorescence and immunohistochemistry assays.
Martin SL. Ribonucleoprotein Particles with LINE-1 RNA in Mouse Embryonal Carcinoma Cells. Molecular and Cellular Biology. 1991; 11(9):4804-7.
Shoji M, Tanaka T, et al. The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline. 2009; 17(6):775-87.