CU researchers have developed a polyclonal antibody that recognizes human p50RhoGAP, which activates Rho-type guanosine triphosphate (GTP) metabolizing proteins. The gene encoding p50RhoGAP protein is ARHGAP1. The antibody has been tested and recognizes dog, rat, and mouse p50RhoGAP protein. Researchers have confirmed that the antibody can be used in western blotting, ELISA, flow cytometry, immunofluorescence, immunoprecipitation, and immunohistochemistry assays.
Schiel JA. Simon GC, Zaharris C, Weisz J, Castle D, WU CC, and Prekeris R. FIP3-endosome-dependent formation of the secondary ingression mediates ESCRT-III recruitment during cytokinesis. Nature Cell Biology. 2012; 14(10):1068-1078.