A research group led by Dr. Liping Yu have developed multiplex assays which can be used for high throughput, simultaneous screening of T1D and its comorbidities using autoantibodies. These assays can measure up to ten autoantibodies using separate linkers for each labeled antigen and electrochemiluminescence detection. They require a small volume and can be performed with as little as 6ul of blood. The inventors successfully created an 8-plex assay which allowed for screening of T1D, celiac disease, autoimmune thyroiditis, and APS-1 in a single well of a 96 well plate. This multiplex assay was validated using T1D and healthy patient samples and retained 100% sensitivity for all autoantibodies.
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