CU scientists have developed monoclonal antibodies targeting the C-terminus of Six1, which is a transcription factor important for development and formation of the muscle, kidney, inner ear, and other organs. Importantly, the antibody is specific to Six1 and is not cross-reactive with other Six family members. The antibody was generated by immunizing rabbits with a C-terminal peptide that corresponds to the region from nucleotide 822 to the stop codon of full-length human Six1 cDNA. Investigators have confirmed that the antibody can be used in western blotting, immunocytochemistry, and immunohistochemistry.
Qamar L, Deitsch E, Partick AN, et al. Specificity and prognostic validation of a polyclonal antibody to detect Six1 homeoprotein in ovarian cancer. Gynecologic Oncology. 2012; 125(2):451-7